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| Reverse-iTTM One-Step Kit. Unique, highly-sensitive RTase Blend containing RNase inhibitor. One-step RT-PCR: minimises reagent handling steps and reduces the risk of contamination. 2x Master Mix format containing MgCl2 and dNTPs. Convenient ReddyMixTM format means no need to add loading dye (optional). Control template and primers (optional). Store below 0°C, preferably at -20°C, in a constant temperature freezer for up to 1 year. Kit Components: -Reverse-iTTM Blend including RNase inhibitor. -2x RT-PCR Master Mix with final reaction concentrations as follows: -Thermoprime Plus: -0.025 units/µl (=1.25 units in a 50µl reaction) -One-Step Reaction Buffer: -1 x Concentration -dAPT, dCTP, dGTP, dTTP: -200µM of each -MgCl2: 1.5mM. |
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