| Reverse-iTTM Hot-Start kit. It also has superior dynamic range to that of either AMV or MMuLV, making accurate quantitative RT-PCR possible. The kit can be used to detect from as little as 500 femtograms of total RNA. Unique, highly-sensitive RTase Blend containing RNase inhibitor. Built-in hot start for highest specificit. Contains Thermo-Start® DNA Polymerase. Convenient one-step Master Mix format. Easy one-tube set-up. Control template and primers (optional). Kit Components: Reverse-iTTM Blend including RNase inhibitor 2x RT-PCR Master Mix with final reaction concentrations as follows: -Thermo-Start® DNA Polymerase: 0.025 units/µl (=1.25 units in a 50µl reaction). Thermo-Start® Reaction Buffer: 1x concentration -dATP, dCTP, dGTP, dTTP: 200µM -MgCl2: 1.5mM. Store below 0°C, preferably at 20°C, in a constant temperature freezer for up to 1 year. |
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