16 MOLECULAR BIOLOGY ClearLine deoxyribonucleotides (dNTPs), PCR grade water and enhancer Part no. Designation Concentration Packaging CHF Deoxyribonucleotides 257743 Mix of dNTPs, solutions of dATP, dCTP, dGTP and DTTp, 25 mM of each 100 mM 8 tubes of 0.5 ml NC - 257744 Mix of dNTPs, solutions of dATP, dCTP, dGTP and DTTp, 10 mM of each 40 mM 2 tubes of 0.5 ml NC - 257746 Set of dNTP, solution of dATP, dCTP, dGTP and DTTp, 100 µM, in separate tubes 100 mM 16 tubes of 0.25 ml NC - 257745 DNTP set, solution of dATP, dCTP, dGTP and DTTp, 25µM, in separate tubes 100 mM 4 tubes of 0.25 ml NC - MgCl2 257741 MgCl2 25 mM 25 mM 10 tubes of 1.5 ml NC - 257742 MgCl2 25 mM 25 mM 3 tubes of 1.5 ml NC - Enhancer PCR 257543 Betaine solution enhancer for complex matrices 5 M 5 tubes of 1 ml NC - PCR grade water 257605 PCR grade ultrapure water - 1 bottle of 1000 ml NC - 257606 PCR grade ultrapure water - 1 bottle of 500 ml NC - 257607 PCR grade ultrapure water - 6 tubes of 5 ml NC - Part no. Designation Concentration Packaging CHF 257757 10X standard buffer, 1.7mM MgCl2 for Hot Disco Taq and Disco Taq DNA polymerase 10X 3 tubes of 1.5 ml NC - 257749 10X ammonium buffer, 1.5 mM MgCl2 Hot Disco Taq and Disco Taq DNA polymerase 10X 3 tubes of 1.5 ml NC - 257750 10X combined buffer, 1.5 mMMgCl2 Hot Disco Taq and Disco Taq DNA polymerase 10X 3 tubes of 1.5 ml NC - 257755 GC-rich matrix buffer 1 4X Hot Disco Taq DNA polymerase 4X 10 tubes of 1.5 ml NC - 257756 GC-rich matrix buffer 2 4X Hot Disco Taq DNA polymerase 4X 10 tubes of 1.5 ml NC - Buffers for Disco Taq and Hot Disco Taq DNA polymerase Hi-fi Pop DNA polymerase 2 U/µl, with 5X buffer and MgCl2 z Hi-fi Pop DNA polymerase 2 U/µl, with 5X Hi-fi buffer and 25 mM MgCl2 z Amplification of up to 18kb of gDNA and 25kb of DNA λ z To be completed with dNTPs, matrix, primers and water Hi-fi Pop DNA polymerase z For applications that require high fidelity such as cloning, sequencing, SNP analysis and mutagenesis z 60 times more accurate than conventional Taq polymerase z Amplification of long, GC-rich DNA sequences z Complex matrices (very rich in GC, complex secondary structures, very long sequences, etc.) may require the addition of a 5M betaine enhancer (optional) z High elongation rate: 10 sec/kb z 3’ –› 5' exonuclease activity Hi-fi Pop DNA polymerase master mix 2X, ready to use z Master mix ready to use, addition of matrix, primers and water only z Amplification of up to 11 kb of gDNA z The 2X buffer contains Hi-fi Taq DNA polymerase, dNTPs, Hi-fi buffer and 3 mM MgCl2 for a final concentration of 1.5 mM Designation No. of units / no. of 50 µl reactions 5X Hi-fi buffer and 25 mM MgCl2 CHF Hi-fi Pop DNA polymerase 2 U/µl 100 257651 NC - 500 257653 NC - 2500 257650 NC - 5000 257652 NC - Designation Number of 50 µl reactions Number of 20 µl reactions Part no. CHF Hi-fi Pop DNA polymerase Master mix 2X 100 250 257654 NC - 500 1250 257656 NC - 2500 6250 257655 NC - 5000 12500 257657 NC -
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