Artificial tumours® (3D cultures) from tumour cell lines widely used in oncology • More closely mimics the microenvironment of avascular tumours in vivo than 2D cultures • Increasingly involved in the development of medicines • Ideal study solution for aggressive triple-negative breast cancer
The Opti3D kit® includes artificial tumours® produced in OptiPASS® culture medium in 96-well microplates (one tumour per well) • Transport in anoxia and at 4 °C for dormancy until use (alternative to freezing) • Each microplate includes 100 ml of suitable culture medium (OptiPASS® OPTI03) for in vitro testing
Benefits • Direct use of tumours after deconditioning • Reproducible tumour size and morphology • Guaranteed: less than 10% variation in size within and between microplates • Once deconditioned, resumption of tumour growth for a minimum of 10 days • Available for triple-negative breast cancer, very aggressive subtype: MDA-MB-231 line, SUM1315 line • Ideal for screening, toxicity testing or basic research into mechanisms of interest
OptiPASS® serum-free and chemically defined media can be used for the culture of tumour cells or healthy cells, in monolayers (2D culture) and for 3D culture (spheroids, organoids) of a wide range of tumour types of interest in preclinical research.