12 MOLECULAR BIOLOGY Disco taq DNA polymerase Disco taq 5 U/µl, with or without buffer and MgCl2 Disco taq and Disco taq Fever master mix 2X, ready to use z High throughput and reliable amplification for routine applications, screening or high-throughput screening z Amplification up to 5 kb, compatible with TA-cloning and dUTP incorporation z 5' -> 3' exonuclease activity and absence of 3'-> 5' exonuclease activity z Disco taq polymerase 5 U/µl, with or without 10X buffer and MgCl2 z Complete with dNTPs, the matrix, primers, water, 10X buffer and MgCl2 if not supplied z Several stamps available z Master mix ready to use, addition of matrix, primers and water only z The Disco taq 2X master mix contains Disco taq DNA polymerase, NH4+ buffer, 0.4 mM of each dNTP, MgCl2 at a final concentration of 1.5 or 2 mM, and stabilising agents z The Disco taq Fever 2X master mix is supplied with a Fever buffer, for amplifications of up to 4kb with increased specificity, even with difficult templates Available in a "ready to load" version, containing a loading buffer and a red dye to monitor migration: + Can be deposited directly after PCR on an agarose or SDS page gel + No interference with PCR, the products obtained can be used for downstream applications (sequencing, etc.) after purification on a column such as PureIT Exo PCR ClearUp Specificity Optimisation of MgCl2 concentration and required hybridisation temperature - Standard buffer (Tris-HCl pH 8.5, KCl, 15 mM MgCl2, 1% Tween 20) +++ Yes - Ammonium buffer (Tris-HCl pH 8.5, (NH4)2S04, 15 mM MgCl2, 1% Tween 20) + No - Combination buffer (Tris-HCl, pH 8.7, KCl, (NH4)2SO4, 15 mM MgCl2, 1% Tween 20) ++ Depending on the matrix and primers Designation Number of 50 µl reactions Without buffer and without MgCl2 CHF 10X standard buffer and final 25 mM MgCl2 CHF 10X ammonium buffer and final 25 mM MgCl2 CHF 10X combined buffer and final mM MgCl2 CHF Part no. Part no. Part no. Part no. Disco taq 5 U/µl 500 257776 NC - 257604 NC - 257548 NC - 258332 NC - 1000 257777 NC - 257593 NC - 257545 NC - 257592 NC - 2500 257778 NC - 257597 NC - 257546 NC - 257596 NC - 5000 257779 NC - 257600 NC - 257547 NC - 257599 NC - Designation Number of 50 µl reactions Number of 20 µl reactions Final MgCl2 concentration: 1.5 mM CHF Final MgCl2 concentration: 2 mM CHF Part no. Part no. Disco taq master mix 2X 100 250 257553 NC - 257563 NC - 500 1250 257555 NC - 257565 NC - 2500 6250 257554 NC - 257564 NC - 5000 12500 257556 NC - 257566 NC - Disco taq Fever master mix 2X 100 250 257549 NC - - - 500 1250 257551 NC - - - 2500 6250 257550 NC - - - 5000 12500 257552 NC - - - Disco taq master mix 2X, ready to load 100 250 257557 NC - 257567 NC - 500 1250 257561 NC - 257571 NC - 2500 6250 257560 NC - 257570 NC - 5000 12500 257562 NC - 257572 NC - Samples available, contact us
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