13 MOLECULAR BIOLOGY Disco Taq without glycerol z Disco taq 5 U/µl or 50 U/µl with glycerol-free storage buffer, with or without 10X buffer and 25 mM MgCl2 z For applications involving a freeze-drying step, or requiring precise pipetting of small volumes (manual or via a pipetting machine) z 1U allows an average of 500 reactions to be carried out z Several stamps available Specificity Optimisation of MgCl2 concentration and required hybridisation temperature - Standard buffer (Tris-HCl pH 8.5, KCl, 15 mM MgCl2, 1% Tween 20) +++ Yes - Ammonium buffer (Tris-HCl pH 8.5, (NH4)2S04, 15 mM MgCl2, 1% Tween 20) + No Designation Number of units (U) Without buffer and without MgCl2 CHF 10X standard buffer and final 25 mM MgCl2 CHF 10X ammonium buffer and final 25 mM MgCl2 CHF Part no. Part no. Part no. Disco taq 5 U/µl, glycerol-free 500 257590 NC - 257587 NC - 257585 NC - 1000 257588 NC - 257578 NC - 257579 NC - 2500 257780 NC - 257581 NC - 257781 NC - 5000 257589 NC - 257584 NC - 257582 NC - Disco taq 50U/µl, glycerol-free 25000 257575 NC - - - - - 250000 257574 NC - - - - - 2000000 257573 NC - - - - - Disco taq DNA polymerase (continued)
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