041-6621737 · Posibeads™ G4S Linker Positive Control Beads - 250 μl

Posibeads™ provide a unique positive control to confirm specific binding and fluorescence of your antibody conjugate of interest. Posibeads™ behave like cells in suspension, and can be carried through immunostaining and wash steps following the same procedure as your cell-based samples. The 5 nm non-fluorescent polystyrene beads are coated with G4S linker peptide. When incubated in the presence of a fluorescent antibody conjugate targeting G4S linker, the antibody will bind to the beads and result in increased fluorescence relative to unlabeled beads. Unlike compensation beads, the binding is driven by the antibody specifically targeting its antigen. Because of this, these Posibeads™ can be used as a specific control for a G4S linker antibody even when tested as part of a panel of other conjugated antibodies.

Posibeads™ are reproducibly generated and can also be used as a control to enable comparison of fluorescence across multiple instruments, sites, timepoints, and experiments. They can be used as a separate standalone sample or added to a sample of interest to serve as an internal positive control verifying the addition and functional activity of the conjugated antibody. Posibeads™ are not known to bind to any cell types, but a lower number of beads is recommended when mixing with cells to reduce the likelihood of acquiring concurrent or overlapping cell and bead events. -