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Chemicals Chemicals Applichem

Applichem

DNA-ExitusPlus and DNA-ExitusPlus IF - RNase-ExitusPlus

DNA-ExitusPlus: decontamination in 10 minutes at 20 °C or more. For the decontamination of surfaces, appliances, plastics and glassware as well as pipettes. Simply apply the product on the surface to be decontaminated or immerse the containers into the decontaminant product. Instructions supplied with the product. Made from biodegradable compounds. Includes a color indicator to easily visualise the surface area covered with the reagent. DNA ExitusPlusIF is almost without color.

RNase-ExitusPlus: non-alkaline, non-corrosive and non-carcinogenic cleaning solution that is highly active against RNase contamination. Stable for around 18 months and heat resistant. Biodegradable compounds, non-toxic. Mineral acids and alkaline substances are not harmful to the equipment even after prolonged incubation times. A temperature of about 50 ° C will increase the rate of reaction. Store at room temperature.

Solutions and powders for the preparation of electrophoresis gel

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Agarose Basic

• For the separation of DNA and RNA fragments and may be used at concentrations between 0.8 and 2 %

Applichem reagents

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Components of various reaction mixtures

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Biochemistry products for migration buffers

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Ethidium bromide

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Recombinant K Proteinase

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Proteinase K solution

• Endopeptidase of vegetable origin (Tritirachium album) of the serine protease family
• Cuts peptide bonds preferably at the carboxyl group of a hydrophobic side-chain amino acid (e. g. leucine)
• Used in nucleic acid preparations to remove protein contaminants or inactivate DNases and RNases
• Contains glycerol: 40 % (vol/vol)
• Available in 1, 5 and 10 ml sizes
• Also available in lyophilized version

Ready-to-use buffer solutions

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DNA-dye NonTox

• Non-hazardous, non-mutagenic and non-toxic alternative to ethidium bromide
• Ready-to-use 6X charge buffer for direct mixing with samples
• Contains 3 migration dyes: bromophenol blue, xylene cyanol and orange G
• Excitable by UV and blue light
• Excitation/emission spectrum: 495 (pic 1), 260 (pic 2) nm / 540 nm

MARKER VI Pre-colored protein markers from 10 to 245 kDA

• 12 different sized markers between 10 - 245 kDa
• The proteins are coupled with a green chromophore at 25kDa and red at 75kDa
• For SDS-PAGE gels
• Delivered in a ready-to-use load buffer
• Shelf life: 3 months at 2 - 8 ° C and 24 months at - 20 ° C
• Total volume: 500 µl
• Use 3 to 5 µl for mini-gels or 1.5 to 2.5 µl for western transfers; And more for gels greater than 1.5 mm

PCR tested water, RNase- and DNase-free

• Bacterial DNA: not detectable (min. 40 PCR cycles)
• DNases/RNases: not detectable
• In 1.7 ml tubes

Yeast extract

• For use in bacterial growth media
• Lyophilized extract prepared from autolyzed Saccharomyces cerevisiae yeast cells, grown on beet molasses
• Salt-free, dust-free product
• Solubility: in a solution at 10 °C, in deionized water at 40 °C

Cell proliferation - XTT kit

Kit for the quantification of cell proliferation and viability, without the use of radioactive isotopes. Reduction of XTT by cell mitonchondria, appearance of an orange colour.

DAPI method

• Binds to DNA
• Excitation 365 nm
• Bright blue fluorescence 450 - 490 nm
• Fluorescent microscopy, flow cytometry

Mycoplasma qPCR test kit

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4K acrylamide and bisacrylamide solution and TGS buffer for protein electrophoresis

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DNA dye for DNAzure blue gel

• Dye visible to the naked eye after 5 to 30 minutes of exposure to light
• Ultrasensitive, band visualization up to 1 ng of DNA
• Excision of the bands of interest without the use of a UV lamp
• Dye compatible with subsequent use of DNA for sequencing and cloning
• The use of bromophenol blue (found in some loading buffers) is not recommended, it is preferable to use G
• Picture taking possible without imager

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